mouse monoclonal IgG1; Rb Antibody (IF8) is an IgG1 κ mouse monoclonal Rb antibody (also designated retinoblastoma (RB) transcriptional corepressor 1 antibody or RB1 antibody) that detects the Rb protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Rb Antibody (IF8) is available as both the non-conjugated anti-Rb antibody form, as well as multiple conjugated forms of anti-Rb antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, D2, D3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements.