mouse monoclonal IgG2a; p16 Antibody (F-12) is an IgG2a κ mouse monoclonal p16 INK4A antibody (also designated CDKN2A antibody, p16INK4a antibody or p16 antibody) that detects the p16 INK4A protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p16 INK4A Antibody (F-12) is available as both the non-conjugated anti-p16 INK4A antibody form, as well as multiple conjugated forms of anti-p16 INK4A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible protein p21 Waf1/Cip1 (also designated p21, WAF1 or Cip1), Kip1 p27 and p16 INK4A. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has been shown that p16 INK4A binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 INK4A exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.