mouse monoclonal IgG1; p130 Cas Antibody (35B.1A4) is an IgG1 κ mouse monoclonal p130 Cas antibody (also designated BCAR1 Scaffold Protein antibody, Cas Family Member antibody, Cas Scaffolding Protein Family Member 1 antibody, CASS1 antibody, CAS antibody, Breast Cancer Anti-Estrogen Resistance Protein 1 antibody, BCAR1 Cas Family Scaffold Protein antibody, P130Cas antibody, CRKAS antibody, Breast Cancer Anti-Estrogen Resistance 1 antibody, Crk-Associated Substrate P130Cas antibody, CRK-Associated Substrate antibody, P130cas antibody, or CAS1 antibody) that detects the p130 Cas protein of mouse, rat and human origin by WB, IP, IF and IHC(P). p130 Cas Antibody (35B.1A4) is available as both the non-conjugated anti-p130 Cas antibody form, as well as multiple conjugated forms of anti-p130 Cas antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. p130 represents one of several known substrates for v-Crk encoded p47. p130 Cas (for Crk-associated substrate) exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.