mouse monoclonal IgG1; cyclin E Antibody (HE12) is an IgG1 κ mouse monoclonal cyclin E antibody that detects cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by WB, IP, IF and IHC(P). cyclin E Antibody (HE12) is available as both conjugated and non-conjugated anti-cyclin E antibody forms. Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. Cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.