mouse monoclonal IgG2a; DNA Ligase IV Antibody (D-8) is an IgG2a κ mouse monoclonal DNA Ligase IV antibody (also designated LIG4 antibody, DNLI4 antibody, Polydeoxyribonucleotide Synthase [ATP] 4 antibody, Ligase IV DNA ATP-Dependent antibody, Polynucleotide Ligase antibody, DNA Repair Enzyme antibody, DNA Joinase antibody, Sealase antibody, or LIG4S antibody) that detects the DNA Ligase IV protein of mouse, rat and human origin by WB, IP, IF and ELISA. DNA Ligase IV Antibody (D-8) is available as both the non-conjugated anti-DNA Ligase IV antibody form, as well as multiple conjugated forms of anti-DNA Ligase IV antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.