mouse monoclonal IgG1; Pals1 Antibody (G-5) is an IgG1 κ mouse monoclonal Pals1 antibody (also designated Pals1 antibody) that detects the Pals1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. Pals1 Antibody (G-5) is available as both the non-conjugated anti-Pals1 antibody form, as well as multiple conjugated forms of anti-Pals1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Two highly conserved complexes are responsible for the assembly of tight junctions, the Crumbs-Pals1-Patj complex and the Cdc42-Par6-Par3-aPKC complex. Tight junctions assist in the formation of polarity in the epithelia by establishing a barrier to separate apical and basolateral membranes. Pals1, importantly, mediates interaction between the two complexes via interaction with Par6. Loss of Pals1 function results in delayed polarization, decreased transepithelial electrical resistance and an inability to form lumenal cysts. Because tumors exhibit perturbations in epithelial polarity, Pals1 presents a new potential target in the study of carcinogenesis.