mouse monoclonal IgG2a; SAP 61 Antibody (H-3) is an IgG2a κ mouse monoclonal SAP 61 antibody (also designated SAP 61 antibody) that detects the SAP 61 protein of mouse, rat and human origin by WB, IP, IF and ELISA. SAP 61 Antibody (H-3) is available as both the non-conjugated anti-SAP 61 antibody form, as well as multiple conjugated forms of anti-SAP 61 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. SAP 61, also known as SF3A3 (splicing factor 3A subunit 3), PRP9, PRPF9 or SF3a60, is a 501 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A3 family. Localized to the nucleus, SAP 61 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the third subunit of the complex, SAP 61 interacts with subunit 1 (SAP 114) via its N-terminus, while simultaneously binding to 15S U2 snRNP via its zinc finger domain. As is the case for all SF3A subunits, SAP 61 is essential for prespliceosome assembly and cell viability. In addition, a pseudogene exists for SAP 61 on chromosome 20.