mouse monoclonal IgG2b; Chk2 Antibody (A-12) is an IgG2b κ mouse monoclonal Chk2 antibody (also designated Chk2 antibody or CHEK2 antibody) that detects Chk2 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. Chk2 Antibody (A-12) is available as the non-conjugated anti-Chk2 antibody form, as well as multiple conjugated forms of anti-Chk2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.