mouse monoclonal IgG1; MAD2 Antibody (107-276-3) is an IgG1 κ mouse monoclonal MAD2 antibody (also designated MAD2 antibody or MAD2L1 antibody) that detects MAD2 of mouse, rat and human origin by IP, IF and ELISA. MAD2 Antibody (107-276-3) is available as the non-conjugated anti-MAD2 antibody form, as well as multiple conjugated forms of anti-MAD2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.