rat monoclonal IgG2a; Fibroblast Marker Antibody (ER-TR7) is an IgG2a rat monoclonal Fibroblast Marker antibody (also designated Fibroblast Cell Marker antibody, Fibroblast antibody, pan-Fibroblast antibody, or Fibroblast lineage antibody) that detects the Fibroblast Marker protein of mouse, rat and human origin by WB, IP, IF and FCM. Fibroblast Marker Antibody (ER-TR7) is available as both the non-conjugated anti-Fibroblast Marker antibody form, as well as multiple conjugated forms of anti-Fibroblast Marker antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Fibroblast cells synthesize and maintain the extracellular matrix in a wide variety of mammal tissues. Fibroblasts provide a structural framework for many tissues and also play an important role in wound healing. They are continuously secreting precursors of the extracellular matrix, specifically the collagens, glycosaminoglycans, reticular and elastic fibers and glycoproteins. Fibroblasts are morphologically heterogeneous and are not restricted by a polarizing attachment to a basal lamina. Fibroblasts have a branched cytoplasm surrounding an elliptical, speckled nucleus having one or two nucleoli. Fibroblasts proliferate easily, making them a popular cell type for cell cultures in biological research. Fibroblast markers can aid in the identification and behavioral analysis of these cells. The intermediate filament protein Vimentin, for example, is expressed on Fibroblast cells, and it is used as a marker to distinguish the mesodermal origin of the cells.