mouse monoclonal IgG2b; MyD88 Antibody (E-11) is an IgG2b κ mouse monoclonal MyD88 antibody (also designated innate immune signal transduction adaptor antibody) that detects the MyD88 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. MyD88 Antibody (E-11) is available as both the non-conjugated anti-MyD88 antibody form, as well as multiple conjugated forms of anti-MyD88 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Interleukin-1 (IL-1)-induced activation of the NFκB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.