mouse monoclonal IgG2a; PKC Antibody (MC5) is an IgG2a κ mouse monoclonal PKC antibody (also designated PKC antibody or pan PKC antibody) that detects PKC α, PKC β and PKC γ of mouse, rat and human origin by WB, IP, IF and IHC(P). PKC Antibody (MC5) is available as the non-conjugated anti-PKC antibody form, as well as multiple conjugated forms of anti-PKC antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, µ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.