Proteins: are essential nutrients for the human body. They are one of the building blocks of body tissue and can also serve as a fuel source. As a fuel, proteins provide as much energy density as carbohydrates: 4 kcal per gram; in contrast, lipids provide 9 kcal per gram.
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HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系,可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料,以及使用293或CHO细胞时需要考虑的主要差异。...
ExpiCHO是一种高效的瞬时表达系统,可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用,获得CHO表达蛋白必须构建稳定的细胞株,这一过程不仅耗时耗力,而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白,往往只能采用293细胞的瞬转表达系统,但该系统产量低,表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...
Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统,目前已广泛应用于治疗性候选药物的早期鉴定,诊断性试剂的快速生产,以及疫苗研发、结构生物学和膜蛋白生物学等研究领域,成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达,但HEK293 细胞和 CHO 细胞之间存在的内在...
Cell line screening studies require highly efficient protocols for studying many samples in parallel. We have developed a lyse-and-go protocol for digital gene expression profiling of u...
Triple-negative breast cancer (TNBC) has poor prognosis with frequent relapses and deaths using current standard of care treatments. Metabolic reprograming is now recognized as a fundam...