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Protein
Proteins: are essential nutrients for the human body. They are one of the building blocks of body tissue and can also serve as a fuel source. As a fuel, proteins provide as much energy density as carbohydrates: 4 kcal per gram; in contrast, lipids provide 9 kcal per gram.
MAY 17, 2018 | 5:00 AM
Many research laboratories use the ExpiCHO Expression System to transiently produce candidate biomolecules for biologics development. However, after the lead candidate is found, a stable cell...
MAY 16, 2018 | 11:00 PM
摘要杆状病毒表达载体系统（BEVS）是重组蛋白生产的主要平台之一。与哺乳动物表达系统早已转换为无血清，化学成分限定的培养基不同，昆虫表达系统的创新相对较少。昆虫细胞继续依赖成分不限定的含酵母提取物的培养基，而这些培养基可以显示出明显的细胞生长和蛋白质表达的批次差异。在这里，我们介绍一种新型的Sf9细胞杆状病毒表达系统的开发。此系统包括支持高细胞密度、化学成分限定的培养基，...
MAY 16, 2018 | 10:00 PM
HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系，可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料，以及使用293或CHO细胞时需要考虑的主要差异。...
MAY 16, 2018 | 8:00 PM
ExpiCHO是一种高效的瞬时表达系统，可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用，获得CHO表达蛋白必须构建稳定的细胞株，这一过程不仅耗时耗力，而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白，往往只能采用293细胞的瞬转表达系统，但该系统产量低，表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...
MAY 16, 2018 | 7:00 PM
Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统，目前已广泛应用于治疗性候选药物的早期鉴定，诊断性试剂的快速生产，以及疫苗研发、结构生物学和膜蛋白生物学等研究领域，成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达，但HEK293 细胞和 CHO 细胞之间存在的内在...
MAY 10, 2018 | 10:30 AM
Cell line screening studies require highly efficient protocols for studying many samples in parallel. We have developed a lyse-and-go protocol for digital gene expression profiling of u...
MAY 10, 2018 | 9:00 AM
Triple-negative breast cancer (TNBC) has poor prognosis with frequent relapses and deaths using current standard of care treatments. Metabolic reprograming is now recognized as a fundam...
