The ExpiCHO and Expi293 transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate drug identification, reagent production, structural biology and vaccine research and membrane protein biology. While both Expi systems offer the ability to generate high levels of recombinant proteins, the inherent differences between HEK293 cells and CHO cells make these systems differentially applicable for various protein expression needs. Here, we present the latest data on the ExpiCHO and Expi293 Expression systems as well as suggested paradigms for instances where either Expi293 or ExpiCHO would be most applicable for a given research requirement

Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统，目前已广泛应用于治疗性候选药物的早期鉴定，诊断性试剂的快速生产，以及疫苗研发、结构生物学和膜蛋白生物学等研究领域，成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达，但HEK293 细胞和 CHO 细胞之间存在的内在差异，决定了它们对应各种不同的蛋白质表达需求有各自的适应性。在今天的报告中我们将与同道们分享ExpiCHO和Expi293两大系统研发和应用的最新进展，以及一些具有建设性意义的范例，以期帮助广大用户在特定的研究需求下知晓如何从ExpiCHO 和Expi293中选择最适用的瞬时重组蛋白表达系统。