The isolation and expansion of rare and ultra-rare cell populations are important for advancing cell therapy manufacturing. For example, Foxp3-positive naïve regulatory T cells (nTregs) - which play a key role in the induction of self-tolerance and the control of autoimmunity–may account for only 0.5 percent of a sample containing one billion leukocytes, making it extremely challenging to isolate these cells with high purity.
The CGX10 Cell Isolation System from Sony Biotechnology is a powerful closed cell sorter designed for extremely fast isolation of ultra-rare cells with high purity. The system’s Enrichment Mode offers rapid target cell isolation from highly concentrated samples–processing up to 100,000 events per second–while Purity Mode can achieve a purity of up to 97% while sorting cells at up to 15,000 events per second.
Our latest application note details a groundbreaking approach using these modes in tandem for sterile, high throughput isolation of Foxp3-positive nTregs on the CGX10 Cell Isolation System. This step-by-step protocol saves time and resources by bypassing bead-based pre-enrichment steps, proceeding directly to flow cytometry-based cell isolation for efficient cell therapy manufacturing.
Key benefits of the CGX10 Cell Isolation System for sorting ultra-rare populations of cells:
GMP-compliant – for cell and gene therapy manufacturing environments
Efficient sorting – enriches and purifies nTregs from one billion leukocytes in less than five hours
Enrichment Mode – suitable for pre-purifying rare and ultra-rare cell populations (<0.5% target in starting sample)
Purity Mode – achieves isolation with 97% purity
Tandem sorting protocol – enables isolation of highly pure, viable, and expandable nTregs
Sterile – enables reusing the same Sterile Tubing Kit to achieve high final purity
Efficient sorting directly from leukopaks – removes the need for pre-enrichment steps
Read our application note to learn more about sorting ultra-rare T cells directly from leukopaks using the CGX10 Cell Isolation System.
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Sony Biotechnology Inc. is dedicated to helping researchers working across different life science disciplines to achieve the best scientific results. By leveraging Sony's vast know-how in electronics innovation and design we offer next-generation cell analysis systems to accelerate your discoveries. Our goal is to bring a unique perspective to the flow cytometry tools required for in-depth single cell isolation and analysis. With our core expertise in automation and software development we hope to enable discovery research across immunology, oncology, cell biology and microbiology.
