While the introduction of atmospheric pressure ion sources, such as electrospray ionization, revolutionized the field of mass spectrometry it also introduced new instrumental compilations not often considered in modern instrumentation. In most instruments, the amount of ions sampled by the mass spectrometer from the electrospray source is on the order of a few percent. Furthermore, the motion of charged species in vacuum is well understood, yet there remain many unresolved questions about the behavior of microdroplets and ions at atmospheric pressure. Recent observations have shown that ions and charged droplets produced via electrospray ionization can be focused to spot sizes of < 1 mm using only DC electric fields under ambient conditions. In this work, fundamental studies on the focusing and transmission of ions at atmospheric pressure are presented. Work using the Agilent Ultivo triple quadrupole coupled to a 3D printed focusing device reveals mobility dependent sensitivity improvements for a wide range of ions on a miniaturized platform. Additional applications of ion focusing will also be discussed.

Structures for Lossless Ion Manipulations (SLIM) is a technology that provides a means to trap and transmit ions with high efficiency over very long path lengths (up to multiple kilometers) under partial vacuum (1-4 torr) using compact printed circuit board assemblies. This capability enables the generation of ultra-high resolution ion mobility separations due to gas phase interactions of analyte ions as they traverse the extended path length of the device. As the exclusive licensee of this technology out of Pacific Northwest National Laboratory, MOBILion Systems is working in collaboration with Agilent Technologies to develop a SLIM-based product that integrates with Agilent QTOF systems. This presentation will provide an overview of the SLIM technology as well as compelling IM-MS data for applications ranging from biotherapeutic glycan profiling to isomeric lipid biomarker detection. For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

Are you interested in Novel Sample Prep and gaining Powerful Insights with Discovery-based LC/MS Workflows? In this session, we will demonstrate a complete untargeted metabolomics workflow from sample prep to data analysis within the context of cancer cell biology.  We describe an innovative cellular metabolite extraction method that “cracks” (lyses) cells and quenches metabolism under room temperature conditions. Both polar metabolite and lipid extracts are then analyzed with the 6546 LC/Q-TOF, a powerful metabolomics platform that offers simultaneous high resolution and extended dynamic range.  Finally, we leverage a full suite of recent software introductions (Lipid Annotator) and enhancements (Profinder, MPP, Quant) to elucidate the molecular response to perturbations in a cancer cell model.

For Research Use Only. Not for use in diagnostic procedures.

Safety and potency testing in the cannabis and hemp industries necessitates analytical systems that include chromatography, mass spectrometry, and real time quantitative polymerase chain reaction (qPCR). Agilent has developed a suite of eMethods for fast and easy implementation in your lab. Agilent eMethods are simple client-side tools that deploy and install pre-built acquisition and data processing methods and reporting templates, pertinent information about consumables, and sample preparation details where applicable. We provide total workflow solutions that include the instrumentation, consumables for sample preparation.

Agilent products and solutions are intended to be used for cannabis quality control and safety testing in laboratories where such use is permitted under state/country law.

The ExD Option, available for both new and existing Agilent instruments, offers superior electron fragmentation in combination with collision induced dissociation (CID) compared to other existing fragmentation technologies. It allows for the far more complete characterization of proteins and glycoproteins with unprecedented accuracy, speed and simplicity. Unlike ETD, the ExD technology can produce higher energy electrons to fragment across more complex ring structures to give better characterization of glycans as well as to provide better mapping of disulfide chains. The ExD technology preserves labile post-translational modifications on large peptides and intact proteins, which allows for the simultaneous quantitation of multiple phosphorylation and glycation sites in bottom-up and middle down proteomic work flows.In addition, ExD induces side chain fragmentation that can differentiate isobaric leucine from isoleucine, isoaspartate from aspartate and other degradation products affecting biopharmaceutical quality. The superior dissociation of native proteins by ExD with retention of post-translational modifications and side chain differentiation opens new opportunities for more complete characterization of complex proteoforms in a highly cost-effective platform.

For Research Use Only. Not for use in diagnostic procedures.

We present a novel workflow for native MS, using a robust and sensitive LC/MS method using standard LC flow for native protein analysis. Most of the native MS analyses are using a nano-electrospray approach, which faces significant challenges in handling as well as throughput.

The workflow comprised the Agilent 1290 Infinity II LC, the AdvanceBio SEC column, the 6545XT AdvanceBio LC/Q-TOF, and MassHunter BioConfirm software. Examples from ligand-binding proteins, MAb’s as well as non-covalent complexes will be shown using standard-flow conditions, demonstrating charge-state distribution previously only observed with nano-spray.

We have also utilized the Ion Mobility Q-TOF system to determine the Collision Cross Section (CCS) values of the native protein molecules and the result for different mAb classes and biosimiliars will be shown.

For Research Use Only. Not for use in diagnostic procedures.

Veterinary drugs are commonly used chemicals to improve the growth and health outcomes of farm animals raised for human consumption. However, improper use of vet-drugs in animal farming can result in accumulation in animal-derived foods, causing adverse effects to its consumers. To combat adverse health effects and protect public health, global regulations define maximum residue limits (MRLs) for vet-drugs in food of animal origin. A major analytical tool for monitoring vet-drug MRLs is the triple quadrupole LC/MS (LC/TQ). However, despite significant advancements in instrumentation, development of a robust and reliable start-to-finish workflow for screening and quantitative analysis is still a challenge due to expertise needed in multiple topics. Generally, a full workflow requires expertise in sample preparation, instrument method development, and knowledge of sample reporting requirements. To address this, a complete and comprehensive LC/TQ analysis workflow solution is debuted. This workflow solution aims to lower the barrier to entry and enhance lab throughput tremendously by providing; pre-defined consumables, sample preparation methods, instrumental analysis methods, and results reporting to produce highly reproducible, robust, and sensitive analysis of 200+ multi-class veterinary drugs in various animal origin food matrices.