PART 2: Utilizing the 6-base genome to investigate the dynamics of enhancer DNA methylation during cellular differentiation

In part 2 of this webinar, Dr. Emily Hodges, assistant professor of biochemistry at Vanderbilt University, will reveal new data that illustrates how the Hodges Lab is utilizing the 6-base genome to investigate the dynamics of enhancer DNA methylation during cellular differentiation.

 

Dr. Hodges will discuss how the advanced 6-base genome solution, duet multiomics solution evoC, provided a multidimensional view that allowed the team to investigate the correlation of methylation state and chromatin accessibility at specific sites across the genome.

 

Specifically:

 

Until recently, we have been measuring global 5hmC with limited success in identifying genomic locations of 5hmC at base pair resolution. With single-base resolution data, we are able to precisely identify changes at individual gene enhancers, providing a detailed molecular understanding of how these changes impact gene regulatory programming. -Dr. Emily Hodges

 

Dr. Hodges research specialty is DNA methylation and non-coding gene regulatory variation in evolution, development, and disease.

 

The combinatorial genetic and epigenetic technology provides single-base-resolution sequencing data that reveals standard four-base sequencing (A, G, C, and T), and distinguishes between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), on the same DNA molecule. This added resolution will allow greater power in detecting disease associations, identifying novel biomarkers, developing disease classifiers, and monitoring the progression of disease.