An adaptable, automated workflow for increased throughput of full-length RNA, single-cell RNA, and 16S rRNA sequencing

Join this webinar to learn how the Bioscience Core Lab at KAUST has automated their PacBio Kinnex library prep workflow on Opentrons robots to facilitate high-throughput RNA sequencing. Angel Angelov will present an adaptable workflow to automate the Kinnex PCR step for the PacBio Kinnex full-length RNA, single-cell RNA, and 16S rRNA protocols. The workflow includes a Shiny web app where the user can modify various protocol parameters and a python protocol template to execute the protocol on the OT-2 robot. With the release of the newest Opentrons app these parameters can also be modified at runtime. Sequencing data from example Kinnex 16S rRNA and full-length RNA runs as well as points for troubleshooting the protocols will be presented.

 

The PacBio Kinnex kits utilize the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase. Kinnex kits can be used for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems to enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.

 

Opentrons lab robots support automation of techniques important for genomics applications, such as nucleic acid extraction and NGS library prep. With modular pipettes, and on-deck modules, including a Thermocycler and Magnetic Block, users can fully automate their library prep workflows.

 

Learning Objectives