Adeno-Associated Virus (AAV) Capsid Protein Characterization Using LC-Orbitrap MS Technology

Adeno-associated viral (AAV) vectors have emerged as the leading gene delivery platform for gene therapy.  Capsid proteins play an important role in cell binding, internalization, and trafficking, however conventional AAV capsid protein characterization methods such as ELISA and SDS-PAGE are time consuming and do not provide direct viral capsid identification information.

In order to successfully characterize AAV viral proteins one can take advantage of a Thermo Scientific™ Orbitrap™ mass analyzer which provides ultra-high resolution and sensitivity, enabling excellent quality of MS and MS2 data for full AAV capsid protein characterization through intact, top- down and peptide mapping approaches. You are invited to join us to learn how AAV capsid proteins and associated PTMs can be characterized rapidly, even at very low concentration levels, using an LC-Orbitrap MS platform.