From Bench to Bedside: Targeted Gene Editing

CRISPR/Cas gene editing technology has rapidly evolved over the last decade. Its versatility extends from creating targeted research-use disease models in cells and animals, to high-throughput screening applications, to clinical reagents for cell-based therapies. The choice of CRISPR reagents is integral to the efficiency and outcome of your gene editing experiments. The specificity and precision of CRISPR tools have evolved from generic WT variants to high-fidelity variants, single base editors, and prime editors. The formats for these tools have also evolved from plasmids to ribonucleoprotein complexes and one-part, modified synthetic guides. In this webinar, we’ll discuss how next generation tools, such as PURedit™ CRISPR reagents, can take your research from discovery to the clinic. We’ll provide an overview of the important characteristics of a gene editing experiment to consider when choosing CRISPR reagents for your translational experiments, such as off-target effects and quality grades.
 
Topics include:
- Current and novel approaches to targeted gene editing
- CRISPR tools required for targeted gene editing workflows
- Use of PURedit™ CRISPR reagents in a variety of applications to provide enhanced specificity without sacrificing cutting activity
 
Learning Objectives:

1. Explain the key requirements of CRISPR reagents for preclinical and translational research.

2. Discuss performance and quality characteristics of gene editing reagents that make them ideal for preclinical research.

3. Choosing the right CRISPR reagents for your workflow and application.