Centrifugal Elutriation- Utility in the Flow Cytometry Laboratory

Centrifugal elutriation is a mature technology that has been used for purification of cell subsets such as monocytes based upon cell density and size, without the need for labeling. The technique has been widely used for synchronization of cells or to purify cells in distinct cell cycle phases. The specialized elutriation rotor allows loading material into the spinning elutriator chamber, and by varying the fluid flow rate introduced into the chamber to counter the centrifugal force, fractions of particles with predictable size can be removed.

This webinar will review the basic technology, and then illustrate various established applications of centrifugal elutriation. Previously reported applications, as well as new applications under development in our lab, are a logical adjunct to the cell sorting techniques offered in a flow cytometry laboratory.

Learning Objectives:

• Review the basic centrifugal elutriation technology
• Illustrate various established applications of centrifugal elutriation, including reported applications, as well as new applications under development in our lab