Evaluation of MICRONAUT-S Anaerobes MIC broth microdilution panels with a large collection of clinical samples

Susceptibility profiles of anaerobes to antimicrobials have become unpredictable, thus reliable and user-friendly methods for routine susceptibility testing are increasingly needed. The MICRONAUT-S Anaerobes MIC test plate is a commercially available broth microdilution method, suitable for routine clinical microbiology testing. We analyzed a collection of 300 consecutive relevant clinical isolates, including 149 gram-positive and 151 gram-negative strains. The performance of the MICRONAUT-S Anaerobes MIC plate was compared to that of a gradient diffusion method (our laboratory standard), calculating the categorical and essential agreement, as well as the discrepancy rate. All the strains included in this study (with the exception of 1 Porphyromonas isolate) successfully grew in the MICRONAUT-S Anaerobes MIC plate (73% after 24 h of incubation). Overall, essential and category agreements resulted in >95% and >97% respectively, and only a low rate of errors was observed. Beyond the very good analytical performance, several technical advantages in comparison with the gradient diffusion method were observed, that contribute to make the MICRONAUT-S Anaerobes panels suitable for an easy implementation into laboratory routine.

 

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