Expanding Proteomic Horizons with Single-Molecule Proteome and Proteoform Analysis

The field of proteomics is undergoing a revolution to better quantify proteins and unlock the dynamic and valuable source of functional biological insight held in the proteome. Single-molecule protein analysis is key to enabling a wide range of advancements in proteomics, including increased sensitivity, reproducibility, and scale. We are working to deliver comprehensive coverage of the proteome, including resolution of proteoforms.  We have built novel biochemistry for single, intact protein molecule immobilization, instrumentation for highly sensitive iterative probing of those protein molecules with a diverse range of affinity-reagents, and machine learning for data interpretation. Simulations using multi-affinity probes that bind short (2-4 amino acid) epitopes show that the accumulated information from multiple rounds of detection enables identification of more than 95% of the human proteome with just 300 probes. Join the webinar to see the latest data demonstrating protein quantification using Protein Identification by Short-epitope Mapping (PrISM) and early platform performance data that will pave the way to conquering the proteome. Additionally, we will share data quantifying proteoform abundance, including those of highly modified proteins such as tau, using standard commercially available affinity reagents to demonstrate an additional application of our single-molecule analysis platform that has the potential to impact biological research, and ultimately human health.

 

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