Expansion of Human Stem Cells in BioBLU Single-Use Vessels from Eppendorf

DATE: December 6, 2016
TIME: 11:00am PT, 2:00pm ET

Experimentation involving human stem cells (HSCs) is one of the fastest growing fields in research and development due, in large part, to its potential for revolutionizing human disease treatment.  There are multiple HSC platforms being investigated, including human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs). Both have distinct advantages and disadvantages, but what they share is a bottleneck at the stage of scale-up, or the ability to produce enough cells for downstream R&D purposes. In this study, we discuss the successful growth of HSCs, both hMSC and hiPSC, in BioBLU® single-use stirred-tank bioreactors from the small (0.3 L) to bench (3 L) scale. In particular, we highlight the production of one billion hMSCs in a single bioreactor, and the culture of hiPSC at the small scale in parallel bioreactors. 

Learning Objectives:

• Learn about the challenges involved and the differences between culturing MSC and iPSC in bioreactors.
• Learn about culturing stem cells in single-use vessels.