Exploring the art behind protein transfer

Western blotting is a dependable, trusted method for protein detection used by thousands of labs worldwide. While the process of western blotting often seems straightforward, different variables used within the workflow can sometimes create a time consuming and complex system. A critical and instrumental step in the western workflow is the transfer of proteins from gel to membrane. Over the past several years, many different methods and devices have been introduced to help improve, accelerate and reduce hands-on time of the transfer step, regardless of the gel chemistry, membrane type or protein of interest involved. In this session we will discuss the key aspects and benefits of three primary western blotting transfer technologies: traditional wet (tank) transfer, semi-dry transfer and dry transfer.