High-Throughput qPCR Microfluidics Technology With Singleplex Accuracy for Plant and Animal genomics

Problem

Screening greater than 10 pathogens or SNPs in a sample is challenging due to limitations in fluorescence colors in a multiplex quantitative PCR. Optimizing a multiplex qPCR is daunting, and it limits addition or removal of assays without optimizing again.

 

Solution

Microfluidics qPCR technology allows massively parallel reactions using a single color with singleplex accuracy. The sensitivity and sample input volume are comparable to those of a standard thermal cycler workflow. Miniaturization leads to cost saving, simplified high-throughput workflow, and efficient reaction kinetics.

 

Proof

The sensitivity of microfluidics qPCR technology is comparable to that of a standard qPCR thermal cycler. It can generate 9,216 qPCR results in 4 hours.

 

Learning Objectives