Impact of Novel Laminar Wash™ on Nuclei Retention and Other Downstream Applications - A Cerevance Story

This talk aims to describe a popular workflow that profiles specific brain cell types in healthy and diseased post-mortem brain tissues. This extensive procedure consists of tissue homogenization with nuclei extraction, fixation, and antibody staining followed by fluorescence-activated sorting of nuclei. Cell type-specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses. Cerevance's breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings.