Keynote Presentation: High-yield Vesicle Packaged Recombinant Protein Production from E. Coli with Live Q&A

During my research career I have worked to further understanding of how intracellular organisation changes in response to the needs and environment of the living cell, focusing on the regulation and function of the actin and myosin cytoskeleton. Since joining the University of Kent School of Biosciences with a BBSRC David Phillips Research Fellowship, my research group has used molecular-genetics, biochemical and live-cell imaging techniques to study the regulation, mechanism and function of dynamic cellular components in growing and dividing cells. Using unicellular yeast and bacterial model systems, we have gained insights into universal mechanisms and regulatory processes of cell growth and division. Over recent years I have applied this expertise in microbial cell biology to develop a number of recombinant technologies for use in biotechnology applications. These bacterial technologies, which include recombinant amino-terminal acetylation, microcompartment utilisation and more recently recombinant vesicle production, are a main focus of my group's current research activities.

Learning Objectives:

1. Define what the challenges are in the expression of recombinant proteins in a cell.

2. List the advantages of using bacteria to express recombinant proteins.

3. Discuss what the current limitations are of using bacteria to produce recombinant protein.