Measuring Immune Cell Killing Dynamics through Real Time Impedance and Live Cell Imaging

The cytolytic activity of Immune cells toward cancer cells is finely regulated through several pathways and receptors. Although end point assays have been routinely used to measure cell killing, they have intrinsic limitations which led to new in vitro potency assays that are more sensitive and better predictive of in vivo outcomes. The introduction of kinetic approaches has tremendously expanded the dynamic range and resolution of potency determination, allowing the development of safer drugs. In this webinar, Dr. Cerignoli will provide an overview of the Agilent xCELLigence Real Time Cell Analysis technology and its utility in cell mediated cytolysis.  He will describe the advantages and limitations of impedance and imaging alone approaches and highlight the importance of comprehensive multiparametric analysis.

For Research Use Only. Not for diagnostic procedures.

Learning Objectives:

1. Comprehend advantages of combining real time impedance and live cell imaging in your immuno-oncology workflow

2. Identify Important factors to consider when designing cell-mediated cytolysis xCELLigence experiments 3. Understand specific cellular phenomena from kinetic data analyzed with RTCA Software