Methods of Single Vesicle Analysis and Considerations for their Use in Quantifying Extracellular Vesicles

The explosion of interest in extracellular vesicles has largely coincided with the development of tools which allow their rapid and routine characterization, in this presentation we will identify and discuss the fundamental principles behind several common tools used for quantifying individual EVs and consider what limitations and strengths should be recognized in each technique. With this understanding we will walk through example data comparing bulk and single particle measurements to develop a better understanding of the real composition of EVs. Lastly we will consider which techniques should be selected for a given investigation based on the research question at hand. 

Learning Objectives:

1. Identify the subtle differences in techniques for detecting single nanoparticles.

2. Review how the choice of a particular technique can influence the collection of the data.

3. Explain how to choose the appropriate technique for their work based on the research question under investigation.