Microscale TFF with µPULSE: A Novel Solution for Lab-Scale Concentration, Buffer Exchange, and Desalting

In this presentation, we will present a novel way of concentrating samples and performing buffer exchange using the µPULSE —TFF System. The instrument is a fully automated walk-away system that uses miniaturized tangential flow filtration (TFF).

Walk-Away System: Set your desired parameters and let the system do 95% of the process automatically until it is time to collect your sample. You can remotely monitor progress via a web-based application.

Fast: Permeate flow rate up to 2.5x faster than centrifuges with dead-end filters.

Scalable Process: Whether you are trying to scale up or down, µPulse allows you to control all the parameters of an industrial TFF at the research scale.

Wide Range of Operating Volumes: Concentrate from 100 mL to 1 mL with up to 250 mL of buffer exchange.

Lowest Hold-up Volume in the Industry: Our patent-pending pump design offers just 0.65 mL of hold-up volume—up to 100% hold-up recovery with automated recovery options.

Easy Maintenance: All components in the fluid path are disposable consumables.

Cost-Effective: The µPulse consumable chips can be cleaned in place for re-use up to 300 mL of permeate (sample dependent).

Learning Objectives: 

• Understand the principles behind miniaturized TFF with µPulse
• Demonstrate the time and reagent-saving benefits of our technology
• Discuss the versatility of the applications and features of the system