Multiplex detection of oncogenic mutations using LNA-based assays on the QIAcuity digital PCR system

Multiplex detection of oncogenic mutations using LNA-based assays on the QIAcuity digital PCR system Digital PCR (dPCR) enables specific and sensitive detection of genetic alterations in oncogenes. It is particularly suited for the analysis of rare mutations in precious samples such as circulating cell-free DNA (ccfDNA) from blood and other body fluids. For reliable detection of single base exchanges at low frequency, both, a reproducibly working dPCR system and optimized PCR assays, are indispensable. Locked Nucleic Acid (LNA)-enhanced oligonucleotides with substantially high levels of affinity for their complementary sequences make these assays excellent tools for the detection and discrimination of highly similar DNA targets. Here, we discuss simultaneous, high-sensitivity detection of up to three different mutations using QIAGEN’s QIAcuity dPCR system in combination with the dPCR LNA Mutation Assays.

Learning Objectives:

1. Learn how to detect simultaneous, high-sensitivity detection of up to three different mutations 

2. Learn how the QIAcuity DPCR System works

3. Learn what are dPCR LNA mutations assays