RNASeq has become the primary method for studying changes in gene expression in modern genomics research. By directly sequencing the various types of RNA molecules, including mRNA, ncRNA, and small RNA, researchers can build an accurate profile of gene expression under various conditions, allowing the elucidation of gene function and regulation. RNASeq has been further refined recently with the development of stranded RNASeq, which modifies the existing RNASeq library construction methodology to preserve information regarding which DNA strand the RNA transcript originated from. Among other advantages, stranded RNASeq allows for more accurate mapping of RNASeq transcripts to reference genomes, which in turn results in a more accurate view of the transcriptome. Increasingly, researchers are designing more complex experiments involving population level transcriptomic surveys and time course studies, both of which require high throughput RNASeq library construction methods that consistently generate high quality RNASeq libraries. In response, we developed an automated RNASeq library construction method using the Beckman Coulter Biomek FXP liquid handling system in conjunction with TruSeq Stranded mRNA library preparation chemistry from Illumina. By combining Illumina's proven chemistry with the highly efficient Biomek FXP liquid handling system, we have developed a flexible library construction protocol that allows for the creation of up to 96 individually indexed Illumina stranded mRNA libraries by a single technician over the course of approximately 8.5 hours of machine time.