Practical considerations for fluorescence microscopy

In this presentation, we will go over some of the reasons you would want to consider using fluorescence imaging and give a brief introduction to using fluorescent probes for cell structure and organelle dynamics; basic cellular function including cell cycle, endocytosis and signaling; and finally, processes involved in cell stress and death such as apoptosis, autophagy, and oxidative stress. We will also go over fluorescent labeling tips and tricks from the Molecular Probes™ School of Fluorescence Basic Imaging Module. Part of getting good results is reviewing your less-than-perfect outcomes and figuring out what to try next time. You will learn about some of the problems you may encounter, see examples of what it looks like when things go wrong, and get hints for preventing those same problems in the future.