Revolutionizing targeted small molecule clinical quantitation using the Thermo Scientific Stellar mass spectrometer

Developing and validating RUO targeted quantitative methods for clinical applications presents many challenges to researchers. Resulting LC-MSⁿ methods cover diverse molecular classes of compounds, from amino acids to endogenous peptides. The resulting methods must meet established limits of detection and quantitation (LOD and LOQ, respectively) in the presence of complex biological matrices. Although the set of molecules composing a panel may be small (< 50), the panel must be validated over a wide dynamic range. Lastly, the resulting data must also meet rigorous confirmatory requirements.

 

The Thermo Scientific™ Stellar™ mass spectrometer is an ideal instrument to overcome the many challenges facing researchers. The Stellar mass spectrometer has a quadrupole mass filter, dual-purpose ion concentrating routing multipole, and dual-pressure linear ion trap that expands effective data acquisition schemes to maintain the quantitative sensitivity for both large and small molecules. This webinar will cover method development for three different applications, including amino acids and acylcarnitines using fast chromatography, highly sensitive steroid hormones, and endogenous peptide quantitation. The webinar will showcase how high- and low-energy collisional dissociation using tMS2 and tMS3 acquisition improves quantitative selectivity, sensitivity, and specificity in complex biological matrices.

 

Learning Objectives