Ultraviolet Photodissociation of Small Molecules: Impurity Analysis and Unknown Identification

Impurities in products may give rise to toxicities, but often represent only minor components. Thus the ability to detect these low abundant analytes in the presence of higher amounts of product is a challenge. Once detected, the correct structure assignment to the impurity or degradation by-products is the next hurdle. Here, learn how Ultraviolet Photodissociation (UVPD) can be utilized in the analysis of impurities as a unique fragmentation mode for overall improved analysis of unknowns. UVPD combined with the high sensitivity and wide dynamic range of the Thermo Scientific™ Orbitrap™ Tribrid™ mass spectrometer provides unique structurally diagnostic information. This otherwise unobtainable information improves the certainty of identification.