Protein Binding: Protein binding can enhance or detract from a drug's performance. As a general rule, agents that are minimally protein bound penetrate tissue better than those that are highly bound, but they are excreted much faster. Among drugs that are less than 80-85 percent protein bound, differences appear to be of slight clinical importance. Agents that are highly protein bound may, however, differ markedly from those that are minimally bound in terms of tissue penetration and half-life. Drugs may bind to a wide variety of plasma proteins, including albumin. If the percentage of protein-bound drug is greater when measured in human blood than in a simple albumin solution, the clinician should suspect that the agent may be bound in vivo to one of these "minority" plasma proteins. The concentration of several plasma proteins can be altered by many factors, including stress, surgery, liver or kidney dysfunction, and pregnancy. In such circumstances, free drug concentrations are a more accurate index of clinical effect than are total concentrations. Formulary committees must grasp the clinical significance of qualitative and quantitative differences in protein binding when evaluating competing agents.
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SPRm (Surface Plasmon Resonance Microscopy) is a novel technology that enables label-free and real-time measurements of the binding affinity and kinetics of small molecules to G protein-coup...
AUG 10, 2023 | 8:00 AM
C.E. CREDITS
Antibody therapeutics is a fast-growing market boosted by developments in bispecific antibodies and in newly emerging tetra-specific antibodies. With the ability to recruit a combination of...
JUL 26, 2023 | 2:30 PM
C.E. CREDITS
Phosphorylation of α-synuclein at the Serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states,...
JUL 26, 2023 | 12:15 PM
C.E. CREDITS
Genetically modified cell models accelerate the development of safe and effective therapeutics, making them indispensable in the quest for new drugs. Through manipulation of cell line geneti...
Affinite Instruments is a developer and supplier of SPR analysis instruments, related consumables, and software. Focusing on the mission of SPR for everyone, this webinar will review the ben...
FEB 22, 2023 | 7:30 AM
C.E. CREDITS
In general, once a receptor drug target has been validated for the treatment of a particular condition, either a high throughput screen is carried out to identify potential hits, or existing...
FEB 22, 2023 | 5:30 AM
C.E. CREDITS
In this project, we optimized a high-throughput fluorescent-based assay to assess transporter activity by measuring uptake of the substrate, ASP+ ï€ 4-(4-(dimethylamino)styryl)-N-methylpyridi...
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
Date: January 25, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) The field of proteomics is poised for a single-molecule revolution – enabling more comprehensive analysis of the...
Date: November 30 Time: 11:00am (PDT), 2:00pm (EDT), Kidney function is assessed in the laboratory by measuring serum creatinine (Cr) and/or......
Date: November 08, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) While flow cytometry shines by its flexibility, it requires a high level of expertise in antibody panel development,...
Date: October 13, 2022 Time: 10:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Most known immune checkpoints exert their effects upon protein-protein interactions, such as the PD1 checkpoint on CD...
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using its chara...
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