Experience with Rapid NGS in solid tumor genomic profiling

Next-generation sequencing (NGS) has transformed solid tumor molecular diagnostics by enabling comprehensive genomic profiling. However, traditional NGS workflows are often labor-intensive and time-consuming, which can delay clinical decision-making. To overcome these challenges, a new automated and rapid molecular profiling approach was validated for formalin-fixed, paraffin-embedded (FFPE) tumor samples across various solid tumor types. This workflow, encompassing library preparation, sequencing, and data analysis, was evaluated for turnaround time, accuracy, reproducibility, and concordance with established NGS platforms. The automated process significantly reduced turnaround time to 4-5 working days, demonstrated high concordance with standard NGS platforms for key actionable variants, minimized manual errors, and improved assay reproducibility. This streamlined approach facilitates decentralized testing, expanding access to biomarker-driven cancer management in diverse settings. Further clinical validation and real-world studies will enhance the utility of rapid NGS workflows in routine oncology diagnostics

 

Learning Objectives